Isolation and characterization of two basal breast cancer model cell lines

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dc.contributor.advisorEwald, Andrew J
dc.contributor.committeeMemberKavran, Jennifer M
dc.creatorGentz, Melissa L
dc.creator.orcid0000-0002-1708-6416
dc.date.accessioned2021-06-25T20:02:44Z
dc.date.available2021-06-25T20:02:44Z
dc.date.created2021-05
dc.date.issued2021-05-06
dc.date.submittedMay 2021
dc.date.updated2021-06-25T20:02:44Z
dc.description.abstractEpithelial-to-mesenchymal transition (EMT) and its reversed process, mesenchymal-to- epithelial transition (MET) remain a topic of debate among the cancer research community. Here, we sought to isolate two novel cell lines from the basal-like mouse models (ROSAmT/mG C3(1)- Tag and C3(1)-Tag) of triple-negative breast cancer (TNBC) to investigate the effects of 2D and 3D culture methods on the expression of epithelial and mesenchymal phenotypes. We demonstrated that co-expression of E-cadherin and vimentin is present in both cell lines when cultured in 2D. Our data also suggests that when embedded in 3D matrices including Rat tail collagen 1 and Matrigel, TGF􏰚 signaling induces spheroid invasion. However, it may play a role in inhibiting colony formation in Matrigel. Taken together, my data reveal that C3(1)-TagM and C3(1)-Tag cell lines may provide researchers with an alternate platform to research hybrid EMT.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://jhir.library.jhu.edu/handle/1774.2/64285
dc.language.isoen_US
dc.publisherJohns Hopkins University
dc.publisher.countryUSA
dc.subjectC3(1)-Tag, triple negative breast cancer, TNBC, basal breast cancer, EMT, MET, vimentin, TGFbeta
dc.titleIsolation and characterization of two basal breast cancer model cell lines
dc.typeThesis
dc.type.materialtext
local.embargo.lift2022-05-01
local.embargo.terms2022-05-01
thesis.degree.departmentBiochemistry, Cellular and Molecular Biology
thesis.degree.disciplineBiochemistry
thesis.degree.grantorJohns Hopkins University
thesis.degree.grantorBloomberg School of Public Health
thesis.degree.levelMasters
thesis.degree.nameSc.M.
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