USING A NOVEL 3D DOUBLE-LAYERED TUMOR SPHEROID SYSTEM TO ASSESSS THE EFFECTS OF PACLITAXEL ON CARTINOMA CELLS

Simple item page
dc.contributor.advisorWirtz, Denis
dc.contributor.committeeMemberGilkes, Daniele
dc.creatorOng, Eric Tu
dc.date.accessioned2019-04-15T03:26:21Z
dc.date.created2017-05
dc.date.issued2017-06-14
dc.date.submittedMay 2017
dc.date.updated2019-04-15T03:26:21Z
dc.description.abstractE-Cadherin has been commonly accepted as a tumor suppressor. The E-Cadherin mediated cell-cell junctions prevent individual carcinoma cells to break apart from primary tumor to disseminate into surrounding tissue. Recently, this conventional wisdom has been challenged by clinical studies on breast cancer patients showing that expression of E-Cadherin is associated with poor prognosis. A recent in vivo study has demonstrated that E-Cadherin expression functionally supports rapid tumor growth that correlates to a poor clinical outcome. However, due to the difficulty to study tumorigenesis in vivo or recapitulate tumorigenicity in vitro, the mechanism by which E-Cadherin precedes tumor growth is largely unknown. In this study, we develop a novel 3D in vitro system in which we successfully assembled matrigel and collagen gel to mimic the structure and juxtaposed organization of the basement membrane and stromal extracellular matrix. Our system supports breast cancer cells to grow, to organize their multicellular structure, to invade through the matrigel layer into the surrounding collagen I matrix and to organize neighboring stromal tissue. Our 3D system allows us to continuously monitor cell proliferation, invasion, collective migration, and tumor growth simultaneously. We utilize the system to observe E-Cadherin expression and paclitaxel response on carcinoma cells. Our current results indicate the presence of E-Cadherin changes multicellular organization and the invasion of cancer cells, leading to faster tumor growth and increasing the half-maximal inhibitory concentration of paclitaxel.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://jhir.library.jhu.edu/handle/1774.2/60426
dc.language.isoen_US
dc.publisherJohns Hopkins University
dc.publisher.countryUSA
dc.subjectE-Cadherin
dc.subject3D in vitro system
dc.subjectPaclitaxel
dc.titleUSING A NOVEL 3D DOUBLE-LAYERED TUMOR SPHEROID SYSTEM TO ASSESSS THE EFFECTS OF PACLITAXEL ON CARTINOMA CELLS
dc.typeThesis
dc.type.materialtext
local.embargo.lift2019-05-01
local.embargo.terms2019-05-01
thesis.degree.departmentChemical and Biomolecular Engineering
thesis.degree.disciplineChemical & Biomolecular Engineering
thesis.degree.grantorJohns Hopkins University
thesis.degree.grantorWhiting School of Engineering
thesis.degree.levelMasters
thesis.degree.nameM.S.E.
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ONG-THESIS-2017.pdf
Size:
2.55 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
LICENSE.txt
Size:
2.67 KB
Format:
Plain Text
Description:
Download
Collections
ETD -- Graduate theses