Isolation and characterization of two basal breast cancer model cell lines
Embargo until
2022-05-01
Date
2021-05-06
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Publisher
Johns Hopkins University
Abstract
Epithelial-to-mesenchymal transition (EMT) and its reversed process, mesenchymal-to- epithelial transition (MET) remain a topic of debate among the cancer research community. Here, we sought to isolate two novel cell lines from the basal-like mouse models (ROSAmT/mG C3(1)- Tag and C3(1)-Tag) of triple-negative breast cancer (TNBC) to investigate the effects of 2D and 3D culture methods on the expression of epithelial and mesenchymal phenotypes. We demonstrated that co-expression of E-cadherin and vimentin is present in both cell lines when cultured in 2D. Our data also suggests that when embedded in 3D matrices including Rat tail collagen 1 and Matrigel, TGF signaling induces spheroid invasion. However, it may play a role in inhibiting colony formation in Matrigel. Taken together, my data reveal that C3(1)-TagM and C3(1)-Tag cell lines may provide researchers with an alternate platform to research hybrid EMT.
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Keywords
C3(1)-Tag, triple negative breast cancer, TNBC, basal breast cancer, EMT, MET, vimentin, TGFbeta