Isolation of bacteria from positive blood cultures by filtration to facilitate rapid identification and antimicrobial susceptibility testing in sepsis diagnostics
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Date
2018-11-27
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Johns Hopkins University
Abstract
Sepsis is a time-sensitive condition in which fast and accurate diagnosis is beneficial to patients. The current gold standard in diagnosing sepsis requires plating a sample from a positive blood culture in order to isolate bacteria for further testing. This sub-cultivation step requires on average 18 hours of incubation to grow a sufficient number of bacteria colonies, therefore introducing a delay to perform downstream tests. However, the replacement of this step with a faster method will allow clinicians to identify the responsible pathogen and administer the appropriate antibiotics sooner. To reduce time to results, a method based upon tangential flow filtration was developed to isolate viable bacteria from a positive blood culture in a matter of minutes. This method produces a bacteria suspension that excludes blood cells and analytes, allowing the sample to be used in subsequent identification and antimicrobial susceptibility testing. By eliminating the subcultivation step, patients can receive optimized treatment earlier, which is correlated with positive clinical outcomes.
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Keywords
Sepsis, bacteria, filtration