Long Range Gene Enhancers Regulate c-Myc RNA through Chromatin Looping in Prostate Cancer
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Date
2016-02-02
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Johns Hopkins University
Abstract
It has been previously shown in a semi-quanitative way that c-Myc confers androgen-independent prostate cancer cell growth, and a significant finding from this work showed AR regulates c-Myc at a posttranscriptional level. The data presented here shows that c-Myc protein expression is required for both normal and prostate cell growth, however c-Myc mRNA and protein is expressed and maintained differently between these two types of cell lines.
Using a new method, SmartFlares, we are able to show from a single cell method without having to extract RNA that c-Myc mRNA remains constant when treated with bicalutamide, while c-Myc protein decreases. We propose that an enhancer 21.7 kb upstream of the c-Myc gene becomes activated and loops to the c-Myc promoter in prostate cancer.
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Keywords
c-Myc, androgen receptor